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Ventral Sexing Thread
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NBond1986
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PostPosted: Thu Dec 06, 2007 4:54 pm    Post subject: Ventral Sexing Thread Reply with quote

Well, ATS has a great thread like this, so I thought I'd start our own:

This is a really great article by Mark R. Hart and Rick West about how to ventrally sex your T, by looking for the presence of epiandrous fusillae.

http://www.birdspiders.com/faq_sex.php
(Need Adobe to read)

I learned a lot by reading this. If you haven't ever read this article and you're serious about T's, I HIGHLY recommend reading it.

I'm still practicing my visual detection techniques.

If you have any side-by-side comarison photos of male/female epigastric furrows, or just individual photos (no comparison necessary) please feel free to post them.

You can also submit photos of unsexed individuals, for the rest of us to see if we can help. Remember, the photos have to be clear, and well lit close ups of the epigastric furrow and surrounding setae/fusillae, in order for us to ventrally sex.

Thanks, and I hope this thread is of great help to us all!
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sick4x4
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PostPosted: Fri Dec 07, 2007 3:09 pm    Post subject: Reply with quote

sexing and sight identifiny pokies...

Female on left, Male on right.
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ftorres
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PostPosted: Sun Dec 09, 2007 12:40 am    Post subject: Po metallica ventral pics Reply with quote

HEllo all,
I have uploaded my ventral pics of the P metallicas. Check them out and tell me what you think.
regards
francisco

Each pic is named as Pmet1 to Pmet5.
there should be about 4-5 pics of each P metallica:

http://s233.photobucket.com/album...o%20metallica%20ventral%20sexing/
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NBond1986
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PostPosted: Mon Dec 10, 2007 10:24 am    Post subject: Reply with quote

Phormictopus sp.
(Originally thought to be P. cubensis, more likely to be P. auratus)

MALE:


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Last edited by NBond1986 on Mon Dec 10, 2007 10:25 am; edited 2 times in total
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NBond1986
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PostPosted: Mon Dec 10, 2007 10:24 am    Post subject: Reply with quote

Crassicrus laminai

FEMALE (the white coloration is just due to lighting)

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NBond1986
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PostPosted: Mon Dec 10, 2007 11:30 am    Post subject: Reply with quote

Francisco, looks like you have 2 males and 3 females.

This is what I think (and I'm 99% sure of 5, 4, 3, and 2. But the Pmet1 pictures aren't very good, so I'm about 75% sure on that one):

Pmet5 is definitely a male.


Pmet4 is a female.


Pmet3 is a female.


Pmet2 is definitely a male.


Pmet1 looks like a female, but the pictures are at an angle, so it's harder to tell. This is the best picture:

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ftorres
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PostPosted: Wed Dec 12, 2007 12:45 am    Post subject: Reply with quote

HEllo All,
Neshan,
You and Wayne agreed on the sex of the following slings:

1  fem
2 male
3 fem
4 no
5 no

more opinios wanted please.
thanks

francisco
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NBond1986
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PostPosted: Wed Dec 12, 2007 9:48 am    Post subject: Reply with quote

I'm still absolutely certain on 4 and 5

On 5, the epiandrous fussillae are really obvious. See the dark arch over the light spot just above the epigastric furrow?

That's a sure sign of a male.

Females don't have the fussillae.

If you look at #4, you will see that she has one continuous color, both anterior and posterior of the epigastric furrow. This is a female.

If anyone wants to chime in with their opinions, please do. This is a good learning experience for everyone.
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sick4x4
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PostPosted: Wed Dec 12, 2007 2:30 pm    Post subject: Reply with quote

sorry neshan only sufusca , metallica and tigirs are exceptions to the rule of vent sexing.....conventional vent techniques don't work for these species..let me find the paper and ill post it....

look at #4 again, there is clearly a bell beginning to show on top of the epigastric furrow, indicating its a male...at this age continuous color isn't a sexual que for this particular species...by the time they reach 3" plus, color can be a que but by that time the bell or lack of it will be prodominatly visible....also feeling is a good indication at 2"s or above...a lump will indicate male and a lack of it female..which is opposite in G.pulchras where the female posses the lump on the furrow and the male does not...

anyways hope that helps..
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NBond1986
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PostPosted: Wed Dec 12, 2007 3:03 pm    Post subject: Reply with quote

sick4x4 wrote:
sorry neshan only sufusca , metallica and tigirs are exceptions to the rule of vent sexing.....conventional vent techniques don't work for these species..let me find the paper and ill post it....

look at #4 again, there is clearly a bell beginning to show on top of the epigastric furrow, indicating its a male...at this age continuous color isn't a sexual que for this particular species...by the time they reach 3" plus, color can be a que but by that time the bell or lack of it will be prodominatly visible....also feeling is a good indication at 2"s or above...a lump will indicate male and a lack of it female..which is opposite in G.pulchras where the female posses the lump on the furrow and the male does not...

anyways hope that helps..


That's an interesting point. There are definitely a few exceptions to the rule, but that doesn't necessarily mean that you can't tell the sex by looking for fussillae.

I wasn't aware that P. metallica was one of the exceptions. Thanks.

Also, the "lump" technique isn't established as a foolproof method, like the fussillae method is. There are many who disagree with it entirely.

The best method, is to just wait, and let them grow Mr. Green
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PostPosted: Wed Dec 12, 2007 3:36 pm    Post subject: Reply with quote

true ::lol::...that's really the only full proof way..buts who's that patient...
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PostPosted: Wed Dec 12, 2007 3:48 pm    Post subject: Reply with quote

P. metallica, P. miranda and P. tigrinawesseli all have bilobed (two "peaks") and fused (connected at the base) spermathecae, as opposed to the remaining Poecilotheria which have unilobed (one "peak) spermathecae. It is not unlikely that they start with bilobed spermatheca as this is a plesiomorph state, which then will fuse to become the typical triangular spermatheca with increasing maturity (ontogenesis). On the other hand what you are seeing could just as well be the male accesory organs that in small slings can be mistaken for a budding spermatheca.

You will have to wait to be sure ;-)
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PostPosted: Fri Jan 25, 2008 12:47 am    Post subject: Reply with quote

Hello All,
Look at this for Pokies

http://www.atshq.org/forum/showthread.php?t=14623




What you guys think???

If this is true and I am not mistaken Nesh is right on sexing my P met and I check them and I agree with him Nesh.

Francisco
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PostPosted: Fri Jan 25, 2008 12:50 am    Post subject: Reply with quote

So here we go again

I need help sexing these P metallicas

P met 5



P met 4




P met 3



P met 2



P met 1 (2 pics)





thank in advance.

Celeste I will be curious to see pics of yours, could it be done????

regards
francisco
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ftorres
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PostPosted: Sat Jan 26, 2008 2:06 am    Post subject: Reply with quote

Here are other Ts that I need help sexing.

B smithi



T blondi



T blondi 2



thank you.

francisco
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PostPosted: Tue Feb 05, 2008 3:17 pm    Post subject: Reply with quote

correct ventral sexing operates on exactly the same principle as correct molt sexing, that is looking for the presence or absence of an actual ~organ.  

when molt sexing you look for spermethecae and/or bursa copulatrix.  if present, female. in a sense, you can only confirm females from molt sexing, as not seeing the organ doesn't necesarily mean it is not there... just that you can't see it.  but... it is not *too* much of a bad assumption at all to assume that on a larger spider if you can't see s/bc then it is probably a male.

when vsexing you are looking for the presence or absence of epiandrous fusilae.  these are ~micro spinnerets that only males have and are used to make sperm webs.  only males have these and males always have them (~mutants not withstanding).  here is the rub... they are tiny. TINY.  i don't look for the individual EF... i look for the field of different looking "hair" that they make.  so in essence you can only confirm males from EF sexing.... if you don't see the EF it doesn't necesarily mean they are not there... only that you can't see them.

there is NO ambiguity in whether the presence of EF indicate a male (or maybe a hermaphrodite).  where i see the ambiguity lying is:
1) not knowing what and where to look for
2) not having perfect focus in the correct spot in pictures
3) using a jpeg image format to post pictures that otherwise would be good enough

1) when i hear ANYTHING other than EF being discussed for vsexing i am immediately cautious. EF is the only thing i know of that has such clearly defined parameters. it must be on males and it must be in quite a specific area.  a *possible* secondary test can be made for increased sclerotization of the epigastric furrow (which indicates female)... but i would consider it at best a secondary confirmation.

2) as i said, individual EF are absolutely tiny.  even the ~characteristic change of the gnap of the setae indicating EF is pretty subtle.  if the camera is not focused extremely well AT THE EXACT RIGHT SPOT or if there is container material between the spider and the lens the important part of the image can not contain enough data to be useful.  

3) jpeg picture format was built by pornagraphers to have a fairly nice looking image with a fairly small file size.  it is a compression format.  it is inherently lossy, to some degree.  when a 2MB 2024x1536 image file is cooked down to a 40kb 400x300 image there is going to be a quite insane loss of actual image data. the result is only a like, approximation of the original... and all fine detail is lost.  that would be the same fine detail you need to look at to successfully EF sex
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There may be people who like centipedes. I have seen people handling tarantulas and scorpions, but never a centipede handler. I would regard such a person with deep suspicion...Now what sort of man or woman or monster would stroke a centipede on its underbelly "And here is my big good centipede." If such a man exists, I say kill him without more ado. He is a traitor to the human race.

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WBurke17
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PostPosted: Tue Feb 05, 2008 3:30 pm    Post subject: Reply with quote

What he'd say Rolling Eyes  Question
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NBond1986
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PostPosted: Tue Feb 05, 2008 3:37 pm    Post subject: Reply with quote

I agree with all but your #2

While there is a lot of truth to what you say there, a picture does not HAVE to be perfect in order to be easily aware of the presence or lack of the EF.

Just my opinion on the subject.

It might depend on the species that you are thinking about.

In some, it's much easier and obvious to tell than in others.

We must take that into consideration.

For instance, M. robustum is known (IIRC) as being one of the more difficult ones to ventrally sex using the EF method.

However, I have always found some other species to be pretty easy, even with an almost blurry picture.
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PostPosted: Tue Feb 05, 2008 7:11 pm    Post subject: Reply with quote

ftorres wrote:
Here are other Ts that I need help sexing.

B smithi



T blondi



T blondi 2



thank you.

francisco


Hey Francisco,

If I were to take a guess I would say that both your B. smithi and your T. blondi are female but I cant tell for certain with the Smithi.

I would bet money that the T. blondi is Female though!
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PostPosted: Tue Feb 05, 2008 10:27 pm    Post subject: Reply with quote

Francisco - agreed w/paradox.
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PostPosted: Tue Feb 05, 2008 11:57 pm    Post subject: Reply with quote

Hello Guys,
Oh man I was hoping for some male blondi, now I have 2 more girls.  Very Happy

I need me to get some males.

francisco
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PostPosted: Wed Feb 06, 2008 1:12 am    Post subject: Reply with quote

you can always hook me up with another female frisco....
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PostPosted: Wed Feb 06, 2008 7:12 am    Post subject: Reply with quote

NBond1986 wrote:
I agree with all but your #2

While there is a lot of truth to what you say there, a picture does not HAVE to be perfect in order to be easily aware of the presence or lack of the EF.

Just my opinion on the subject.

It might depend on the species that you are thinking about.

In some, it's much easier and obvious to tell than in others.

We must take that into consideration.

For instance, M. robustum is known (IIRC) as being one of the more difficult ones to ventrally sex using the EF method.

However, I have always found some other species to be pretty easy, even with an almost blurry picture.


well, i mean for really obvious species and individuals, sure... but it is easy to say your picture must be clear and in focus all the time rather than make this big complicated list of exceptions =P

i need to get one of those self lighted super magnifiers that get mentioned with regards to vsexing, for sure.
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There may be people who like centipedes. I have seen people handling tarantulas and scorpions, but never a centipede handler. I would regard such a person with deep suspicion...Now what sort of man or woman or monster would stroke a centipede on its underbelly "And here is my big good centipede." If such a man exists, I say kill him without more ado. He is a traitor to the human race.

William S. Burroughs The Western Lands

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sick4x4
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PostPosted: Wed Feb 06, 2008 7:25 am    Post subject: Reply with quote

andrew always the old skooler.... Wink though i agree with you, i also think characteristic que identification also can be accurate..its only major flaws would be pic quality and size of the specimen but this can be elevated with picking up the specimen and flipping it over...even though some genus-es are easier than others using this method. i can only imagine with enough work, it can almost be as accurate as EF...

we have progressed some since the invention of the light ::lol::.......
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PostPosted: Wed Feb 06, 2008 8:00 am    Post subject: Reply with quote

well, i have problems with any method that isn't tied to organ existence checks


i mean... i *just* recently started to get the idea of how to sex off chelicerae.... but compared to an existance check a relative comparison is WAY more fuzzy and prone to error


but... what i do think is that several relative comparison checks that all point to the same sex is a better guess than 50/50 for sure.  ::lol::... and if you are consistantly wrong more often than right... keep guessing the same way and just switch your answer heh



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There may be people who like centipedes. I have seen people handling tarantulas and scorpions, but never a centipede handler. I would regard such a person with deep suspicion...Now what sort of man or woman or monster would stroke a centipede on its underbelly "And here is my big good centipede." If such a man exists, I say kill him without more ado. He is a traitor to the human race.

William S. Burroughs The Western Lands

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